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"Although there was no physical contact, I have regretted my irresponsible behavior. Once again, I ask (the 20-year-old) for his forgiveness, as well as the people of the church." Watch Haggard in the HBO documentary »
Haggard founded New Life in his basement in 1984 and oversaw its growth to an influential megachurch with roughly 15,000 members.
Haggard is the former president of the National Association of Evangelicals, a group that says it represents millions of people in 45,000 church congregations nationwide. He and other evangelical leaders participated in weekly conference calls with senior White House aides during the Bush administration.
In November 2006, allegations surfaced that Haggard had a three-year relationship with Mike Jones, a male escort, involving drugs and sex for money. After those allegations became public, New Life fired Haggard and he resigned as president of the national organization.
On Friday, New Life Church announced Haggard’s relationship with the volunteer in a letter to the congregation.
In the last three weeks, Boyd said, the young man told him that he was considering going public with his story because Haggard was portraying himself as a victim in an upcoming HBO documentary called "The Trial of Ted Haggard," which is scheduled to air Thursday.
"I counseled him it wasn’t going to help him or the church’s healing. Plus it was in violation of the agreement we signed with them," Boyd said.
An interview with the former volunteer is scheduled to air Monday evening on KRDO-TV of Colorado Springs, Colorado. Haggard is scheduled to appear on CNN’s "Larry King Live" on Thursday night.
The 2006 controversy involving Haggard erupted when Jones, the former prostitute, said the pastor had paid him for sex over three years and had used methamphetamine in his presence. Haggard said in interviews that he received a massage from Jones, but denied having sex with him. He also said he bought methamphetamine, but threw it away instead of using it.
The assertions received widespread news coverage and sparked charges of hypocrisy, particularly because Haggard had condemned homosexual sex in a documentary called "Friends of God."
In a settlement with New Life, the church and Haggard agreed that he would retain his six-figure salary for a year, leave the Colorado Springs area, receive counseling, and not speak publicly about what had happened for one year, according to a church staff member with knowledge of the settlement who was not authorized to speak on the record.
Jones said Monday that he has spoken with the former church volunteer whom Boyd said had a relationship with Haggard.
"My anger is so much at the church," Jones said. "They tried to keep this quiet when all along I’ve taken a beating feeling like I was the only one, when clearly there were others."
Despite the fresh scandal, Boyd said he is hopeful for the future.
"We’ve added 1,500 people in the last 18 months," he said. "We’re trying to adopt 100 orphans into families in the church.
"We had a leader with flawed character and we’re trying to clean up from the residue of the past. This will not always define us. I can promise you that in the days ahead, we won’t be identified with scandal, but helping the widows and orphans."
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"To appoint unto them that mourn in Zion, to give unto them beauty for ashes ,�" Now, we use that term even in our English vernacular. What does it mean? To have something that is as unattractive as a pile of ashes suddenly become something as beautiful as a rose in full bloom. What�s the picture? When the world comes out of the horrors of the Tribulation, it�s going to be ashes, but it�s not going to stay that way.
Now, this is a good time to jump up with me to Jeremiah, chapter 25. We�ve used these verses at least in some of our Oklahoma classes. Now, if you�ve got a little imagination whatsoever, you can see that this is the ashes that�s going to be the result of those seven years of wrath and vexation coming on the planet.
Now, I read a lot. I think you all know that. I�m amazed at how many intellectual people, if they know anything of this at all, scorn it. They just ridicule it. There�s nothing bad ever going to happen. They talk in terms of what�s going to be going on in four, five, six hundred years from now. Well, I�ve got news for them. They may be intellectuals. They may have degrees. They may think they�re the elite. But this little old farmer knows a lot more than they do, because I can go according to the Book. This is what�s coming, because this is what your Bible says.
Turn to Jeremiah chapter 25 verse 30. Now, don�t scoff at this. Don�t just shrug this off and say this is just some disgruntled prophet. No, this is inspired by the Spirit of God and even though we�re not going to be here, and we don�t have to worry about it happening to us, yet it�s for us to know to warn people that it�s coming. All I have to say if they have any doubt is aren�t they listening to the news? Aren�t they reading the news? What is the uppermost thought in the nations of the world today? Weapons of mass destruction. That�s all they�re thinking about. That�s all they�re talking about.
Russia has now announced that they�re coming out with a greater nuclear weapon than anything else that�s in the world. Well, what do they hope to do with it? Iran. They want to produce nuclear weapons. What are they going to do with them? Just as soon as somebody drops one anywhere, somebody else is going to retaliate, and it�ll be a domino effect. God isn�t going to allow it to happen. He�s going to do it. But it�s coming. All the weapons of mass destruction are going to be used, but in God�s time. All of these diseases that the world is worried about, they�re coming. All of the extraterrestrial things, it�s all coming, because God is going to make this planet ashes out of which He can bring in the beauty of His Kingdom. All right, here are the ashes.
"Therefore prophesy thou against them all these words, and say unto them, The LORD (Jehovah, Israel�s God) shall roar from on high, and utter his voice from his holy habitation; (Second Coming. When He�s going to return.) He shall mightily roar upon his habitation; (That is this planet.) He shall give a shout, as they that tread the grapes, (Now, I�ll come back to this when we get into the later chapters of Isaiah, probably next month�s taping.) against all the inhabitants of the earth." Not just Israel, but every human being that�s still living will fall under this wrath of a righteous God.
"A noise shall come even to the ends of the earth;�" Now, I don�t know how you interpret that, but I think it�s real simple. Nuclear bombs exploding completely around the planet. Now, you talk about a domino effect. That�s what�s going to happen. As soon as somebody drops a nuclear bomb on one place, somebody else is going to retaliate. It�s going to be, well, like the Book of Revelation puts it, it�s just going to be like a domino effect. The cities of the world are going to disappear. That�s in Revelation 17 and 18. All right, but this is the Old Testament�s view.
"�for the LORD hath a controversy with the nations, (Plural) he will plead with all flesh; he will give them that are wicked to the sword, saith the LORD. (In other words, their life is going to be taken.) 32. Thus saith the LORD of hosts, Behold, evil shall go forth from nation to nation, and a great whirlwind shall be raised up from coasts (borders) of the earth." Now, what�s one of the other effects of nuclear explosion? Tremendous wind.
Remember when they dropped the little atomic bombs in Japan? They were little compared to what we�ve got today. They were just firecrackers, and yet what did they experience? Tremendous winds that even blew existing buildings down. That�s all part of it, the heat and the fire and the wind. All right, so I think this is a perfect description of how it will finally come to its end.
"And the slain of the LORD�" Why? Because He is pouring out His wrath on Christ rejecting mankind. It isn�t that He�s unfair. He has given, now, over 2000 years of grace and mercy, and they still scorn it. My, even in this Age of Grace that we feel is coming to a close, He has made salvation so available. Just by believing in your heart that Jesus died for your sins and rose from the grave as we see in I Corinthians 15:1-4. But most walk that free gift under foot.

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Citation: Songok EM, Luo M, Liang B, Mclaren P, Kaefer N, Apidi W, et al. (2012) Microarray Analysis of HIV Resistant Female Sex Workers Reveal a Gene Expression Signature Pattern Reminiscent of a Lowered Immune Activation State. PLoS ONE 7(1): e30048.
Editor: Clive M. Gray, University of Cape Town, South Africa.
Received: May 17, 2011; Accepted: December 8, 2011; Published: January 26, 2012.
Copyright: � 2012 Songok et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study was funded by Bill and Melinda Gates Foundation under Grand Challenges in Global Health (GCGH) initiative and the Canada Institutes of Health Research (CIHR). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The disease AIDS perhaps ranks as one of the most devastating scourges of mankind. Since it was identified in 1983 more than 30 million people have died and another 33 million currently live with the virus [1], [2]. The majority of HIV-1 infections occur in Sub-Saharan Africa, where in some countries prevalence rates of more than 40% have been documented among antenatal clinic attendees [3]. Despite advances made in antiretroviral therapy, only a third of those requiring treatment receive it and new infections far outstrip those on therapy [4]. Similar to other previous viral epidemics, prevention through vaccination shall be the best approach. However, the nature of the AIDS virus to integrate itself to the host genome and its ability to constantly mutate and shield immunogenic components that induce protective antibodies, continue to pose challenges for an effective vaccine. Though a recent prime boost vaccine strategy, RV 144, provided signals that a HIV-1 vaccine was possible, its efficacy was only a modest 30% [5].
The presence of individuals who are highly exposed to HIV-1 but do not get infected, provide hope for a better understanding of correlates for protection that may lead to a more effective vaccine strategy. Highly exposed seronegative (HESN) populations have been identified among intravenous drug users [6], children born to seropositive mothers [7], [8], discordant couples [9], [10], and commercial sex workers [11]�[13]. The findings in 1996 on the protective effect of the CCR5?32 allele in HIV-1 infection [14] shifted focus to host genetics as probable cause of HIV-1 resistance. Over the past 10 years, approximately 40 genes have been documented from HESN populations as possible candidates for differential host susceptibility to HIV-1 [15]. This include selected human leukocyte antigens, HLA [16], TRIM5? [17], specific KIR-HLA associations [18], and APOBEC3G [19]. However, only a fraction of HESN has the protective genotypes described in the literature. Previous gene discovery studies have focused on single gene approaches which may have missed other compelling gene candidates and overlook interactions between different genes or genetic pathways. Microarray expression profiling provides new opportunities for simultaneous analysis of thousands of messenger RNA expression patterns in tandem with their biological functions. Less data exist on the use of microarrays to identify biomarkers for HIV-1 susceptibility, including pathways that may be critical for successful anti-HIV-1 vaccine and therapeutic design. In a bid to determine biomarkers and genetic pathways that are unique to HESNs, we carried out a global whole blood gene expression profiling of HIV-1 highly exposed and uninfected individuals from a well-characterized female commercial sex worker cohort from Nairobi, Kenya.
Gene expression outcome.
Our primary objective was to investigate if there is a predictive gene expression pattern that defines the HESN phenotype. Gene expression data from the 86 micro arrays were normalized and all were included in the analysis. An unsupervised hierarchical clustering algorithm was run on the data to ascertain if the two populations could be separated into two distinct groups based on their gene expression profiles. Figure 1 show a distinct separation between the HESN (Test) and HIV negative controls (Controls).
Each row on the Y axis represents a single gene probe and the phylograms represent distinct signaling pathways. The red color denotes up regulated genes while the green are down regulated.
Of the 54675 gene probe sets represented on the Affymetrix U133 plus 2.0 gene chip, 2,274 probe sets were differentially expressed in the HESN group as compared with the control group (fold change ?1.3; p value ?0.05) after correction with multiple testing using the Benjamin-Hochberg false discovery rate (FDR<0.05). Of the total differentially expressed transcripts, 462 (20%) could be mapped onto the KEGG signaling database. Figure 2 shows the 15 signaling events with the highest number of genes identified through the KEGG analysis. Majority of these pathways (13 of 15, 87%) were significantly down-regulated in the HESN group. The top ranked down-regulated pathways belonged to energy metabolism, cell adhesion, signal transduction and immune signaling categories. Glucose/gluconeogenesis and pentose phosphate phosphorylation (p<0.0003) were the most down-regulated metabolic pathways, together with the immune signaling events of natural killer cell cytotoxicity (p<0.008), antigen processing and presentation (p<0.0013) and T-cell receptor signaling. The key up-regulated pathways were the ribosome protein synthesis and tight junction. Figures 3 (a to g) show the heatmaps of representative genes in the significantly impacted pathways. Gene ontology (GO) classification of the differentially expressed transcripts identified protein binding as the major functional group among the down-regulated genes (Fig. 4a). In contrast, up-regulated genes were significantly associated with nuclear binding (Fig. 4b).

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